The Mouse HSP-90 (Heat Shock Protein 90) CLIA Kit is specifically designed for the accurate measurement of HSP-90 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.HSP-90 is a chaperone protein that plays a crucial role in protein folding, stability, and cell signaling. It is involved in various cellular processes, including stress response and cell cycle regulation.
Dysregulation of HSP-90 has been implicated in numerous diseases, such as cancer, neurodegenerative disorders, and autoimmune diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse HSP-90 CLIA Kit provides researchers with a reliable tool for investigating the role of HSP-90 in health and disease, offering valuable insights into its biological functions and potential therapeutic targets.
Product Name:
Mouse HSP-90 (Heat Shock Protein 90) CLIA Kit
SKU:
MOES00329
Target:
Mouse HSP-90 (Heat Shock Protein 90)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HSP-90. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HSP-90. You can calculate the concentration of Mouse HSP-90 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
102-114
88-101
Average (%)
100
108
93
1:4
Range (%)
89-101
90-104
98-116
Average (%)
94
95
106
1:8
Range (%)
95-107
87-100
95-111
Average (%)
102
93
101
1:16
Range (%)
85-100
93-106
85-97
Average (%)
91
101
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
100-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
90.96
316.14
837.49
87.78
323.2
825.43
Standard deviation
9.75
29.34
85.84
8.28
30.15
87.66
C V (%)
10.72
9.28
10.25
9.43
9.33
10.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HSP-90 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HSP-90 in samples. No significant cross-reactivity or interference between Mouse HSP-90 and analogues was observed.
