The Mouse Intact Parathormone (PTH) ELISA Kit is specially designed for the quantitative detection of intact parathormone levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures accurate and reliable results for a variety of research applications.Parathormone is a key hormone that regulates calcium and phosphate levels in the body, playing a critical role in bone metabolism and mineral homeostasis.
Dysregulation of parathormone levels can lead to conditions such as hyperparathyroidism and osteoporosis, making it a valuable biomarker for studying these disorders and developing potential interventions.Overall, the Mouse Intact PTH ELISA Kit provides researchers with a powerful tool for investigating parathormone-related pathways and advancing our understanding of bone health and mineral metabolism in mouse models.
Product Name:
Mouse I-PTH (intact Parathormone) ELISA Kit
SKU:
MOES01205
Target:
Mouse I-PTH (intact Parathormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse I-PTH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse I-PTH. You can calculate the concentration of Mouse I-PTH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
97-112
94-105
Average (%)
97
103
99
1:4
Range (%)
91-107
80-94
83-95
Average (%)
98
86
88
1:8
Range (%)
90-105
84-96
85-99
Average (%)
98
89
91
1:16
Range (%)
92-103
88-98
86-102
Average (%)
97
93
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.86
148.59
404.65
42.73
135.98
383.0
Standard deviation
2.6
7.52
14.12
2.53
7.71
18.12
C V (%)
5.67
5.06
3.49
5.92
5.67
4.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse I-PTH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse I-PTH in samples. No significant cross-reactivity or interference between Mouse I-PTH and analogues was observed.
