The Mouse IFABP (FABP2) Intestinal Fatty Acid Binding Protein CLIA Kit is a powerful tool for the quantitative detection of IFABP levels in mouse samples. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.IFABP, also known as FABP2, is a key protein involved in the transport and metabolism of fatty acids in the intestine. Dysregulation of IFABP levels has been implicated in various gastrointestinal disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its user-friendly format and robust performance, the Mouse IFABP Intestinal Fatty Acid Binding Protein CLIA Kit is an indispensable resource for researchers seeking to gain insights into the molecular mechanisms underlying intestinal lipid metabolism.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFABP/FABP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFABP/FABP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFABP/FABP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IFABP/FABP2. You can calculate the concentration of Mouse IFABP/FABP2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
97-112
97-112
Average (%)
99
103
103
1:4
Range (%)
103-116
93-108
89-102
Average (%)
109
100
95
1:8
Range (%)
86-100
88-103
92-106
Average (%)
92
94
97
1:16
Range (%)
86-97
94-109
94-109
Average (%)
91
101
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
101-116
107
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.84
278.79
740.45
109.83
302.01
671.59
Standard deviation
8.73
31.03
48.65
13.72
35.58
62.86
C V (%)
8.25
11.13
6.57
12.49
11.78
9.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IFABP/FABP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IFABP/FABP2 in samples. No significant cross-reactivity or interference between Mouse IFABP/FABP2 and analogues was observed.
