The Mouse IFABP (FABP2) Intestinal Fatty Acid Binding Protein ELISA Kit is specially designed for the accurate measurement of mouse IFABP levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it an ideal tool for various research applications.IFABP, also known as FABP2, plays a crucial role in fatty acid metabolism and transport in the intestines. It is involved in the absorption and transportation of dietary fatty acids, making it a key player in lipid metabolism.
Dysregulation of IFABP levels has been implicated in various gastrointestinal disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Mouse IFABP (FABP2) Intestinal Fatty Acid Binding Protein ELISA Kit provides researchers with a reliable and efficient tool for investigating the role of IFABP in health and disease.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFABP/FABP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFABP/FABP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFABP/FABP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFABP/FABP2. You can calculate the concentration of Mouse IFABP/FABP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
94-109
89-103
Average (%)
104
102
95
1:4
Range (%)
91-105
83-97
83-95
Average (%)
96
90
88
1:8
Range (%)
91-107
85-97
85-96
Average (%)
98
91
90
1:16
Range (%)
94-110
83-95
81-94
Average (%)
100
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
88-101
96
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.36
4.8
0.53
1.28
4.48
Standard deviation
0.03
0.07
0.19
0.03
0.08
0.2
C V (%)
6.12
5.15
3.96
5.66
6.25
4.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IFABP/FABP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IFABP/FABP2 in samples. No significant cross-reactivity or interference between Mouse IFABP/FABP2 and analogues was observed.
