The Mouse IFN-Beta (Interferon-Beta) ELISA Kit is a specialized assay designed for the quantitative detection of interferon-beta levels in mouse serum, plasma, and tissue culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes. Interferon-beta is a key cytokine involved in regulating the immune response, particularly in antiviral defense and inflammatory pathways. Dysregulation of interferon-beta signaling has been implicated in various autoimmune disorders, viral infections, and inflammatory diseases, making it a crucial target for therapeutic interventions.
With the Mouse IFN-Beta ELISA Kit, researchers can accurately measure interferon-beta levels in biological samples, enabling a better understanding of immune responses and potential therapeutic strategies for immune-related diseases. Its reliable performance and ease of use make it a valuable tool for studying the role of interferon-beta in health and disease.
Product Name:
Mouse IFN- beta (Interferon Beta) ELISA Kit
SKU:
MOES00653
Target:
Mouse IFN- beta (Interferon Beta)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-β. You can calculate the concentration of Mouse IFN-β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
94-105
92-104
Average (%)
94
100
99
1:4
Range (%)
94-111
85-98
94-109
Average (%)
102
92
102
1:8
Range (%)
101-113
87-101
94-109
Average (%)
107
93
100
1:16
Range (%)
98-112
83-95
93-103
Average (%)
105
89
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.8
98.0
486.5
50.4
89.4
514.5
Standard deviation
2.5
4.6
18.5
2.5
5.3
26.2
C V (%)
5.23
4.69
3.8
4.96
5.93
5.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IFN-β in samples. No significant cross-reactivity or interference between Mouse IFN-β and analogues was observed.
