Mouse IGFBP-3 (Insulin-like Growth Factor Binding Protein 3) CLIA Kit
The Mouse IGFBP-3 (Insulin-like Growth Factor Binding Protein 3) CLIA Kit is a powerful tool for accurately measuring IGFBP-3 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.IGFBP-3 is a key protein involved in regulating the activity of insulin-like growth factors, which play a crucial role in cell growth and differentiation.
By measuring IGFBP-3 levels, researchers can gain valuable insights into the mechanisms of growth regulation and potential therapies for conditions such as cancer, diabetes, and growth disorders.With its advanced technology and ease of use, the Mouse IGFBP-3 CLIA Kit is an essential tool for researchers studying growth regulation and related disorders in mouse models. Unlock the potential of your research with this innovative kit from Assay Genie.
Product Name:
Mouse IGFBP-3 (Insulin-like Growth Factor Binding Protein 3) CLIA Kit
SKU:
MOES00032
Target:
Mouse IGFBP-3 (Insulin-like Growth Factor Binding Protein 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGFBP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IGFBP-3. You can calculate the concentration of Mouse IGFBP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
84-98
90-105
Average (%)
91
91
96
1:4
Range (%)
102-117
87-102
101-114
Average (%)
109
93
107
1:8
Range (%)
94-108
89-103
102-120
Average (%)
99
95
110
1:16
Range (%)
101-118
100-115
95-112
Average (%)
108
107
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
103
EDTA plasma (n=5)
98-112
105
Cell culture media (n=5)
98-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.21
101.39
472.44
53.64
102.29
473.79
Standard deviation
5.27
8.94
33.97
4.4
8.9
51.07
C V (%)
9.9
8.82
7.19
8.2
8.7
10.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IGFBP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IGFBP-3 in samples. No significant cross-reactivity or interference between Mouse IGFBP-3 and analogues was observed.
