The Mouse IL-1 Beta CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of interleukin-1 beta levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a variety of research applications.Interleukin-1 beta is a key inflammatory cytokine that plays a crucial role in the regulation of immune responses and inflammatory processes.
It is involved in a wide range of physiological and pathological conditions, including inflammatory diseases, autoimmune disorders, and infectious diseases. The ability to accurately measure IL-1 beta levels in mouse samples makes this kit an invaluable tool for studying the role of this cytokine in disease pathogenesis and for evaluating potential therapeutic interventions.
Product Name:
Mouse IL-1 beta CLIA Kit
SKU:
MOES00034
Target:
Mouse IL-1 beta
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-1β. You can calculate the concentration of Mouse IL-1β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
100-114
87-98
Average (%)
96
107
92
1:4
Range (%)
97-112
101-114
85-98
Average (%)
105
107
92
1:8
Range (%)
92-106
93-105
92-104
Average (%)
97
99
98
1:16
Range (%)
101-118
86-99
101-115
Average (%)
108
92
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
99-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.43
50.57
169.33
20.43
50.48
175.12
Standard deviation
2.45
3.72
18.63
2.1
4.24
12.28
C V (%)
11.43
7.36
11.0
10.28
8.4
7.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-1β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-1β in samples. No significant cross-reactivity or interference between Mouse IL-1β and analogues was observed.
