The Mouse IL-15 ELISA Kit is a powerful tool for detecting and quantifying levels of IL-15 in mouse serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit provides accurate and reproducible results for a variety of research applications.IL-15 is a key cytokine involved in the regulation of immune responses, playing a critical role in the activation and proliferation of T cells and natural killer cells. Dysregulation of IL-15 has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and cancer, making it an important target for research and potential therapeutic interventions.
With the Mouse IL-15 ELISA Kit, researchers can gain valuable insights into the mechanisms underlying immune responses and disease pathology, paving the way for novel treatments and diagnostic strategies. Trust in the reliability and precision of this kit to advance your research endeavors in immunology, oncology, and beyond.
Product Name:
Mouse IL-15 ELISA Kit
SKU:
MOES01223
Target:
Mouse IL-15
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-15. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-15, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-15. You can calculate the concentration of Mouse IL-15 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
85-101
91-104
Average (%)
94
92
97
1:4
Range (%)
95-107
86-98
90-100
Average (%)
100
92
95
1:8
Range (%)
93-105
80-94
82-93
Average (%)
98
87
88
1:16
Range (%)
90-103
81-93
84-95
Average (%)
96
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
101
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.86
125.0
493.76
55.05
124.94
477.11
Standard deviation
2.86
5.19
19.31
3.75
6.57
18.32
C V (%)
5.51
4.15
3.91
6.81
5.26
3.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-15 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-15 in samples. No significant cross-reactivity or interference between Mouse IL-15 and analogues was observed.
