The Mouse IL-2 ELISA Kit is specifically designed for the precise measurement of interleukin-2 (IL-2) levels in mouse samples, such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for a variety of research applications.IL-2 is a key cytokine involved in regulating immune responses, playing a vital role in the proliferation and differentiation of T cells.
Dysregulation of IL-2 has been implicated in autoimmune diseases, inflammation, and certain types of cancer, highlighting its importance as a biomarker in understanding these diseases and developing targeted therapies.Overall, the Mouse IL-2 ELISA Kit provides a reliable and efficient means of studying the role of IL-2 in immune function and disease pathogenesis, making it a valuable tool for researchers in the field.
Product Name:
Mouse IL-2 ELISA Kit
SKU:
MOES00661
Target:
Mouse IL-2
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-2. You can calculate the concentration of Mouse IL-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
93-107
93-107
Average (%)
95
100
99
1:4
Range (%)
95-112
81-94
92-106
Average (%)
103
86
98
1:8
Range (%)
97-111
84-97
95-110
Average (%)
105
89
101
1:16
Range (%)
100-117
82-92
91-107
Average (%)
108
87
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49
87.4
385.6
52.4
82.2
365.0
Standard deviation
3.3
3.8
20.4
3.1
4.8
15.7
C V (%)
6.73
4.35
5.29
5.92
5.84
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-2 in samples. No significant cross-reactivity or interference between Mouse IL-2 and analogues was observed.
