The Mouse IL-4 ELISA Kit is specifically designed for the precise quantification of interleukin-4 (IL-4) levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for accurate and consistent results that are crucial for various research purposes. IL-4 is a key cytokine that plays a critical role in regulating immune responses, particularly in the development of Th2 cells and the promotion of antibody production.
Its dysregulation has been linked to various inflammatory and autoimmune diseases, making it a valuable marker for studying these conditions and exploring potential therapeutic approaches. With its user-friendly protocol and reliable performance, the Mouse IL-4 ELISA Kit is an essential tool for researchers seeking to explore the intricate mechanisms of immune modulation and inflammatory pathways in mouse models.
Product Name:
Mouse IL-4 ELISA Kit
SKU:
MOES00662
Target:
Mouse IL-4
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-4. You can calculate the concentration of Mouse IL-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
85-99
89-102
Average (%)
99
91
94
1:4
Range (%)
99-115
87-102
99-113
Average (%)
106
94
105
1:8
Range (%)
99-115
86-101
94-109
Average (%)
107
93
101
1:16
Range (%)
96-111
81-95
96-110
Average (%)
102
87
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
92-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.6
252.2
947.1
99.9
274.5
966.5
Standard deviation
6.5
11.3
30.3
6.4
14.0
36.7
C V (%)
6.66
4.48
3.2
6.41
5.1
3.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-4 in samples. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed.
