The Mouse IL-5 ELISA Kit is a valuable tool for researchers looking to accurately measure interleukin-5 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, guaranteeing precise and consistent results for a variety of research pursuits.Interleukin-5 is a key cytokine involved in the regulation of immune responses, particularly in the differentiation and activation of eosinophils.
It plays a critical role in conditions such as asthma, allergies, and autoimmune diseases, making it a crucial biomarker for studying these conditions and exploring potential treatment options.With its user-friendly design and reliable performance, the Mouse IL-5 ELISA Kit is an essential tool for any researcher looking to delve deeper into the role of interleukin-5 in immune regulation and disease pathology.
Product Name:
Mouse IL-5 ELISA Kit
SKU:
MOES01217
Target:
Mouse IL-5
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-5. You can calculate the concentration of Mouse IL-5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
89-104
90-104
Average (%)
103
95
97
1:4
Range (%)
89-105
79-93
83-96
Average (%)
96
86
89
1:8
Range (%)
93-106
83-98
87-102
Average (%)
99
89
94
1:16
Range (%)
87-100
84-97
84-94
Average (%)
93
91
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.34
86.56
464.22
51.71
92.29
432.78
Standard deviation
2.8
5.01
24.37
3.55
4.08
15.41
C V (%)
5.56
5.79
5.25
6.87
4.42
3.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-5 in samples. No significant cross-reactivity or interference between Mouse IL-5 and analogues was observed.
