The Mouse INHBP (Inhibin Binding Protein) CLIA Kit is a powerful tool for accurately measuring levels of INHBP in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it ideal for a variety of research purposes.INHBP is a key protein that binds to inhibin, a hormone involved in regulating reproductive processes. This protein plays a critical role in various physiological processes and has been implicated in conditions such as infertility and endometriosis.
By studying levels of INHBP, researchers can gain valuable insights into these conditions and potentially develop new treatments.Overall, the Mouse INHBP CLIA Kit offers researchers a reliable and efficient way to study the role of INHBP in health and disease, advancing our understanding of reproductive biology and potentially leading to new therapeutic strategies.
Product Name:
Mouse INHBP (Inhibin Binding Protein) CLIA Kit
SKU:
MOES00385
Target:
Mouse INHBP (Inhibin Binding Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse INHBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INHBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INHBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse INHBP. You can calculate the concentration of Mouse INHBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
88-105
90-106
Average (%)
98
96
98
1:4
Range (%)
97-113
99-113
96-112
Average (%)
103
107
104
1:8
Range (%)
89-104
98-110
100-116
Average (%)
97
104
106
1:16
Range (%)
97-110
88-102
94-110
Average (%)
103
94
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
97-110
103
Cell culture media (n=5)
94-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.55
4.72
0.51
1.42
4.49
Standard deviation
0.05
0.17
0.54
0.04
0.15
0.4
C V (%)
9.8
10.97
11.44
7.84
10.56
8.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse INHBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse INHBP in samples. No significant cross-reactivity or interference between Mouse INHBP and analogues was observed.
