The Mouse INS (Insulin) ELISA Kit by Assay Genie is a specialized assay designed for the quantitative detection and measurement of Insulin levels in various biological samples collected from mouse models.
Insulin is a pivotal hormone involved in the regulation of blood glucose levels, energy storage, metabolism, and various physiological processes. Accurate measurement of Insulin is crucial for studying glucose homeostasis, metabolic functions, and endocrine pathways related to insulin signaling in mouse research models. Our Mouse INS ELISA Kit ensures exceptional sensitivity and specificity, enabling reliable and reproducible results essential for investigating insulin dynamics, metabolic disorders, and potential therapeutic interventions. With stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for research studies focusing on elucidating the role of insulin in mouse physiology and pathogenesis. Trust Assay Genie's Mouse INS ELISA Kit for accurate and dependable quantification of Insulin levels in your mouse research studies.
Product Name:
Mouse INS (Insulin) ELISA Kit
SKU:
AEES00192
Target:
Mouse INS (Insulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 pg/ml
Detection range:
3.13-200 pg/ml
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
88-99
-
Average (%)
93
91
-
1:4
Range (%)
96-110
97-112
-
Average (%)
103
104
-
1:8
Range (%)
94-106
98-107
-
Average (%)
101
101
-
1:16
Range (%)
93-105
88-102
-
Average (%)
99
94
-
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
-
-
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
85-95
nan
90.0
nan
nan
nan
Mean (ng/mL)
20
20.0
20.0
20.0
20.0
20.0
Standard deviation
9.36
24.03
91.53
8.46
24.39
87.8
C V (%)
0.52
1.17
4.07
0.42
1.2
4.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed.
