The Mouse INS (Insulin) ELISA Kit is a specialized assay designed for the quantitative detection of Insulin levels in various biological samples obtained from mice. Insulin is a critical hormone involved in the regulation of blood glucose levels and energy metabolism. It plays a pivotal role in maintaining glucose homeostasis, promoting glucose uptake into cells, and regulating carbohydrate, lipid, and protein metabolism. Accurate measurement of Insulin levels is essential for studying metabolic processes, insulin resistance, diabetes, and related disorders in mouse models.
The Mouse INS ELISA Kit from Assay Genie offers exceptional sensitivity, specificity, and precision, ensuring reliable and reproducible results. Manufactured under stringent quality control conditions, this kit provides robust performance and user-friendly protocols, making it an excellent choice for researchers investigating the role of Insulin in metabolic pathways and disease mechanisms using mouse models. Rely on Assay Genie's Mouse INS ELISA Kit for accurate quantification of Insulin levels in your mouse studies.
Product Name:
Mouse INS (Insulin) ELISA Kit
SKU:
AEES00193
Target:
Mouse INS (Insulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
-
-
93-107
Average (%)
-
-
99
1:4
Range (%)
-
-
96-113
Average (%)
-
-
103
1:8
Range (%)
-
-
96-111
Average (%)
-
-
104
1:16
Range (%)
-
-
97-110
Average (%)
-
-
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
-
-
EDTA plasma (n=5)
-
-
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.3
8.5
1.0
2.3
8.7
Standard deviation
0.1
0.1
0.4
0.1
0.1
0.3
C V (%)
10
4.35
4.71
10.0
4.35
3.45
Sample type &Sample volume:
cell culture supernatant and tissue homogenate; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in cell culture supernatant and tissue homogenate.
Specificity:
This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed.
