Mouse IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) CLIA Kit
The Mouse IP-10 (CXCL10) Interferon-Gamma Induced Protein 10kDa CLIA Kit is specially designed for the accurate and sensitive detection of IP-10 (CXCL10) levels in mouse serum, plasma, and cell culture supernatants. This kit offers high performance and reliability, ensuring precise and consistent results for various research applications.IP-10 (CXCL10) is a key chemokine involved in the immune response, particularly in inflammation and immune cell recruitment. It is induced by interferon-gamma and plays a critical role in the pathogenesis of various inflammatory diseases, making it a valuable biomarker for studying immune responses and developing potential therapies.
Overall, the Mouse IP-10 (CXCL10) CLIA Kit is a powerful tool for researchers studying immune system function, inflammation, and related diseases in mouse models. Its high sensitivity and specificity make it an essential component for accurate and insightful experimental results.
Product Name:
Mouse IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) CLIA Kit
SKU:
MOES00019
Target:
Mouse IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IP-10/CXCL10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IP-10/CXCL10. You can calculate the concentration of Mouse IP-10/CXCL10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-106
98-113
91-105
Average (%)
101
106
97
1:4
Range (%)
100-118
91-103
91-103
Average (%)
107
96
97
1:8
Range (%)
94-109
100-118
103-116
Average (%)
101
107
110
1:16
Range (%)
99-112
90-103
89-100
Average (%)
106
95
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
100
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.41
114.92
324.95
46.74
119.84
304.31
Standard deviation
5.35
12.03
26.97
3.78
10.46
19.02
C V (%)
12.32
10.47
8.3
8.09
8.73
6.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Mouse IP-10/CXCL10 and analogues was observed.
