The Mouse LDL (Low Density Lipoprotein) CLIA Kit is a cutting-edge assay designed for the accurate quantification of LDL levels in mouse serum, plasma, and tissue samples. This kit offers unparalleled sensitivity and specificity, guaranteeing precise and consistent results for a variety of research applications.Low Density Lipoprotein (LDL) is a key player in cholesterol metabolism and is often referred to as the "bad cholesterol" due to its role in the development of cardiovascular diseases such as atherosclerosis.
Monitoring LDL levels in mouse models is essential for studying lipid metabolism, cardiovascular health, and evaluating the efficacy of potential therapeutics.With the Mouse LDL CLIA Kit, researchers can confidently measure LDL levels in mouse samples, providing valuable insight into lipid metabolism and cardiovascular health. Trust in this advanced assay for reliable and reproducible results in your research endeavors.
Product Name:
Mouse LDL (Low Density Lipoprotein) CLIA Kit
SKU:
MOES00617
Target:
Mouse LDL (Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LDL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LDL. You can calculate the concentration of Mouse LDL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
87-99
93-103
Average (%)
108
92
98
1:4
Range (%)
97-107
101-115
95-111
Average (%)
102
107
101
1:8
Range (%)
93-104
96-113
93-106
Average (%)
98
104
99
1:16
Range (%)
88-101
94-110
97-110
Average (%)
93
101
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
107
EDTA plasma (n=5)
97-113
104
Cell culture media (n=5)
96-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.6
11.14
47.79
4.14
10.4
50.26
Standard deviation
0.57
0.86
5.34
0.42
1.24
4.22
C V (%)
12.39
7.72
11.17
10.14
11.92
8.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse LDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LDL in samples. No significant cross-reactivity or interference between Mouse LDL and analogues was observed.
