The Mouse LDL (Low Density Lipoprotein) ELISA Kit is specifically designed for the precise quantification of low-density lipoprotein levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Low-density lipoprotein, commonly known as "bad cholesterol," is a key player in the development of cardiovascular diseases and atherosclerosis. Monitoring LDL levels in animal models is vital for understanding disease progression and evaluating the efficacy of potential therapeutic interventions.
With easy-to-follow instructions and reliable performance, the Mouse LDL ELISA Kit from Assay Genie is an essential tool for researchers studying lipid metabolism, cardiovascular health, and related conditions in mouse models. Order yours today to accelerate your research efforts and advance our understanding of LDL biology.
Product Name:
Mouse LDL (Low Density Lipoprotein) ELISA Kit
SKU:
MOES01674
Target:
Mouse LDL (Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LDL. You can calculate the concentration of Mouse LDL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
96-112
89-102
Average (%)
104
103
96
1:4
Range (%)
101-115
87-100
97-110
Average (%)
107
93
102
1:8
Range (%)
98-116
85-96
96-111
Average (%)
106
91
103
1:16
Range (%)
101-114
81-97
94-108
Average (%)
108
88
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.2
68.5
235.5
24.4
73.1
255.2
Standard deviation
1.4
3.5
10.4
1.5
3.1
8.7
C V (%)
5.34
5.11
4.42
6.15
4.24
3.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse LDL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LDL in samples. No significant cross-reactivity or interference between Mouse LDL and analogues was observed.
