The Mouse Leptin Receptor (LEPR) ELISA Kit is meticulously designed for the precise detection and quantification of leptin receptor levels in various biological samples. The leptin receptor, a key component of the leptin signaling pathway, plays a crucial role in regulating energy balance, metabolism, and appetite. This receptor is involved in mediating the effects of leptin, a hormone primarily produced by adipose tissue that regulates body weight and metabolism. Through the accurate measurement of leptin receptor levels, researchers can gain valuable insights into the mechanisms underlying metabolic homeostasis, obesity, and related metabolic disorders.
The ELISA kit provides exceptional sensitivity and specificity, ensuring reliable and reproducible results that are essential for elucidating the role of the leptin receptor in metabolic regulation. Manufactured under stringent quality control standards, this ELISA kit offers robust performance and ease of use, making it an excellent choice for research focused on understanding the molecular pathways associated with energy balance and metabolic health.
Product Name:
Mouse LEPR (Leptin Receptor) ELISA Kit
SKU:
AEES00234
Target:
Mouse LEPR (Leptin Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEPR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEPR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LEPR. You can calculate the concentration of Mouse LEPR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
95-101
99-109
Average (%)
91
98
105
1:4
Range (%)
97-107
90-104
100-110
Average (%)
101
96
104
1:8
Range (%)
83-95
100-109
90-103
Average (%)
90
103
97
1:16
Range (%)
90-101
92-102
96-105
Average (%)
97
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
97
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
100-106
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.22
23.49
81.49
9.59
22.38
86.39
Standard deviation
0.52
1.08
3.99
0.5
1.06
3.69
C V (%)
5.64
4.6
4.9
5.21
4.74
4.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse LEPR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LEPR in samples. No significant cross-reactivity or interference between Mouse LEPR and analogues was observed.
