The Mouse Low Density Lipoprotein (LDL) ELISA Kit is a comprehensive solution for measuring LDL levels in mouse serum, plasma, and tissue lysate samples. This kit offers exceptional accuracy and precision, allowing researchers to obtain reliable and consistent results for a variety of experimental purposes.LDL, a type of cholesterol-carrying particle, plays a critical role in cardiovascular health and is often associated with the development of atherosclerosis and heart disease in animal models. By quantifying LDL levels in mouse samples, researchers can gain valuable insights into the impact of diet, genetic factors, and experimental treatments on lipid metabolism and cardiovascular health.
With its user-friendly protocol and high-performance components, the Mouse LDL ELISA Kit is an indispensable tool for studies investigating lipid metabolism, cardiovascular diseases, and the effects of pharmacological interventions in mouse models. Trust in the accuracy and sensitivity of this kit to advance your research and discover new insights into the role of LDL in health and disease.
Product Name:
Mouse Low Density Lipoprotein (LDL) ELISA Kit
Product Code:
MODL00788
Size:
96 Assays
Target:
LDL
Detection Method:
Sandwich
Range:
15.6-1,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
