The Mouse LZM (Lysozyme) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of lysozyme levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.Lysozyme is an important enzyme involved in the immune system, with antibacterial properties that help protect the body against infections.
Elevated levels of lysozyme have been associated with inflammatory conditions, making it a valuable biomarker for studying immune responses and disease progression.The Mouse LZM CLIA Kit is a powerful tool for researchers looking to explore the role of lysozyme in health and disease, providing valuable insights and potential therapeutic targets for a range of conditions.
Product Name:
Mouse LZM (Lysozyme) CLIA Kit
SKU:
MOES00429
Target:
Mouse LZM (Lysozyme)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LZM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LZM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LZM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse LZM. You can calculate the concentration of Mouse LZM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
100-113
94-109
Average (%)
105
107
100
1:4
Range (%)
96-108
97-112
99-116
Average (%)
102
104
106
1:8
Range (%)
94-106
99-111
86-99
Average (%)
99
104
92
1:16
Range (%)
97-114
84-95
94-107
Average (%)
105
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.57
272.51
982.44
98.35
249.66
934.72
Standard deviation
11.77
23.68
98.83
11.97
22.44
92.26
C V (%)
10.84
8.69
10.06
12.17
8.99
9.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse LZM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LZM in samples. No significant cross-reactivity or interference between Mouse LZM and analogues was observed.
