Mouse MAPtau (Microtubule Associated Protein Tau/Tau Protein) CLIA Kit
The Mouse MAPTAU (Microtubule-associated protein tau) Protein CLIA Kit is a cutting-edge assay designed for the precise measurement of tau protein levels in mouse samples. This kit offers unparalleled sensitivity and accuracy, providing researchers with reliable and consistent results for a variety of experimental needs. Tau protein is a key player in neurodegenerative diseases such as Alzheimer's and Parkinson's, where abnormal tau accumulation is associated with cognitive decline and neuronal damage.
By accurately quantifying tau levels in mouse models, researchers can gain valuable insights into disease progression and potential therapeutic targets. Whether investigating the mechanisms of tau pathology or evaluating the efficacy of novel treatments, the Mouse MAPTAU Protein CLIA Kit offers a powerful tool for advancing research in neurodegenerative disorders. Trust in its precision and performance to drive meaningful discoveries in the field of neuroscience.
Product Name:
Mouse MAPtau (Microtubule Associated Protein Tau/Tau Protein) CLIA Kit
SKU:
MOES00551
Target:
Mouse MAPtau (Microtubule Associated Protein Tau/Tau Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MAPÏ„. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MAPÏ„ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MAPÏ„, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MAPÏ„. You can calculate the concentration of Mouse MAPÏ„ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
89-105
104-118
Average (%)
93
96
109
1:4
Range (%)
86-100
93-106
88-102
Average (%)
92
99
94
1:8
Range (%)
92-109
93-107
95-109
Average (%)
100
101
103
1:16
Range (%)
85-98
98-110
102-121
Average (%)
91
103
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
103
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.21
61.79
193.84
24.35
59.91
180.84
Standard deviation
2.02
4.67
13.61
2.81
5.76
19.24
C V (%)
8.34
7.56
7.02
11.54
9.61
10.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MAPÏ„ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MAPÏ„ in samples. No significant cross-reactivity or interference between Mouse MAPÏ„ and analogues was observed.
