Mouse MAPtau (Microtubule Associated Protein Tau/Tau Protein) ELISA Kit
The Mouse MapTau (Microtubule-Associated Protein Tau) ELISA Kit is a highly reliable and sensitive tool for the quantitative detection of Tau protein levels in mouse serum, plasma, and tissue homogenates. This kit is specifically designed to provide accurate and reproducible results, making it suitable for a wide range of research applications.Tau protein is a key participant in the regulation of microtubule assembly and stability, playing a crucial role in neuronal function and pathology.
Abnormal accumulation of Tau protein is associated with neurodegenerative disorders such as Alzheimer's disease, making it a valuable target for studying disease mechanisms and potential therapeutic interventions.With its high sensitivity and specificity, the Mouse MapTau ELISA Kit is an essential tool for researchers studying the role of Tau protein in neurodegenerative disorders and other conditions. Get reliable and accurate results with this innovative ELISA kit from Assay Genie.
Product Name:
Mouse MAPtau (Microtubule Associated Protein Tau/Tau Protein) ELISA Kit
SKU:
MOES01529
Target:
Mouse MAPtau (Microtubule Associated Protein Tau/Tau Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MAPτ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MAPτ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MAPτ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MAPτ. You can calculate the concentration of Mouse MAPτ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
90-106
97-114
Average (%)
99
96
104
1:4
Range (%)
88-99
86-98
86-100
Average (%)
94
93
93
1:8
Range (%)
94-108
86-98
85-100
Average (%)
99
91
92
1:16
Range (%)
87-100
82-97
85-97
Average (%)
94
89
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.35
115.9
411.66
52.69
116.07
437.36
Standard deviation
3.79
5.27
20.67
2.71
6.94
13.34
C V (%)
6.97
4.55
5.02
5.14
5.98
3.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MAPÏ„ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MAPÏ„ in samples. No significant cross-reactivity or interference between Mouse MAPÏ„ and analogues was observed.
