The Mouse MBP (Myelin Basic Protein) CLIA Kit is specifically designed for the precise measurement of Myelin Basic Protein levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable tool for researchers in various fields.Myelin Basic Protein is a key component of myelin, which is essential for the proper functioning of the nervous system. Abnormalities in MBP levels have been linked to demyelinating diseases such as multiple sclerosis, making it a crucial biomarker for understanding these conditions and developing potential treatments.
This CLIA kit provides a comprehensive solution for studying the role of Myelin Basic Protein in neurological disorders and other related conditions in mice, allowing researchers to gain valuable insights into disease mechanisms and potential therapeutic interventions.
Product Name:
Mouse MBP (Myelin Basic Protein) CLIA Kit
SKU:
MOES00444
Target:
Mouse MBP (Myelin Basic Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MBP. You can calculate the concentration of Mouse MBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
101-114
86-99
Average (%)
109
108
92
1:4
Range (%)
95-110
89-100
86-100
Average (%)
101
95
91
1:8
Range (%)
90-105
86-101
99-110
Average (%)
96
92
104
1:16
Range (%)
86-100
86-98
103-119
Average (%)
92
91
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
106
EDTA plasma (n=5)
92-105
99
Cell culture media (n=5)
91-107
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.58
279.34
966.85
105.03
254.61
894.57
Standard deviation
12.7
24.89
64.68
11.1
19.1
63.51
C V (%)
11.7
8.91
6.69
10.57
7.5
7.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MBP in samples. No significant cross-reactivity or interference between Mouse MBP and analogues was observed.
