Mouse MCP-2/CCL8 (Monocyte Chemotactic Protein 2) ELISA Kit
The Mouse MCP-2/CCL8 (Monocyte Chemotactic Protein 2) ELISA Kit is a cutting-edge assay meticulously designed for the precise and quantitative determination of MCP-2, which belongs to the monocyte chemotactic protein family, in a variety of biological samples. MCP-2, also known as CCL8, is a chemokine with potent chemoattractant properties that plays a crucial role in immune responses, specifically in the recruitment and activation of monocytes and other immune cells at sites of inflammation or injury. Accurate measurement of MCP-2 levels is essential for comprehending its involvement in the regulation of immune reactions and inflammatory processes.
By quantifying MCP-2 expression, researchers can gain insights into its specific immune-modulating functions, which are fundamental in various inflammatory diseases, immune responses, and pathophysiological conditions where chemokine signaling is implicated. The Mouse MCP-2/CCL8 ELISA Kit crafted by Assay Genie delivers outstanding sensitivity and specificity, guaranteeing precise, reproducible, and dependable results. With stringent quality control measures in place, this kit ensures robust performance and ease of use, making it a top choice for research endeavors that delve into the multifaceted roles of MCP-2 in immune regulation and inflammatory pathways.
Product Name:
Mouse MCP-2/CCL8 (Monocyte Chemotactic Protein 2) ELISA Kit
SKU:
AEES00263
Target:
Mouse MCP-2/CCL8 (Monocyte Chemotactic Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-2/CCL8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-2/CCL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-2/CCL8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MCP-2/CCL8. You can calculate the concentration of Mouse MCP-2/CCL8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
100-111
90-104
Average (%)
91
105
98
1:4
Range (%)
85-98
99-112
91-108
Average (%)
91
104
98
1:8
Range (%)
88-102
100-117
88-102
Average (%)
93
107
94
1:16
Range (%)
86-101
95-108
88-104
Average (%)
92
101
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.03
244.7
742.2
95.79
262.47
748.47
Standard deviation
6.58
9.84
29.39
5.75
11.92
32.03
C V (%)
6.58
4.02
3.96
6.0
4.54
4.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MCP-2/CCL8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MCP-2/CCL8 in samples. No significant cross-reactivity or interference between Mouse MCP-2/CCL8 and analogues was observed.
