Mouse MCP-2 (Monocyte Chemotactic Protein 2) CLIA Kit
The Mouse MCP-2 (Monocyte Chemotactic Protein-2) CLIA Kit is a reliable tool for accurately measuring MCP-2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.MCP-2 is a key chemokine involved in the recruitment and activation of monocytes, playing a critical role in inflammatory responses and immune system regulation.
Dysregulation of MCP-2 has been linked to various inflammatory diseases and immune disorders, making it a valuable marker for studying these conditions and exploring potential therapeutic interventions.Whether investigating immune responses, inflammation, or disease mechanisms, the Mouse MCP-2 CLIA Kit provides a valuable tool for researchers seeking to explore the role of MCP-2 in physiological processes and pathological conditions in mouse models.
Product Name:
Mouse MCP-2 (Monocyte Chemotactic Protein 2) CLIA Kit
SKU:
MOES00439
Target:
Mouse MCP-2 (Monocyte Chemotactic Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-2/CCL8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-2/CCL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-2/CCL8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MCP-2/CCL8. You can calculate the concentration of Mouse MCP-2/CCL8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
87-101
92-108
Average (%)
94
94
99
1:4
Range (%)
101-115
86-97
94-109
Average (%)
109
92
99
1:8
Range (%)
93-104
91-105
89-105
Average (%)
98
97
96
1:16
Range (%)
102-116
102-117
92-106
Average (%)
108
110
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
96-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.22
30.22
69.59
8.47
32.01
66.32
Standard deviation
1.13
2.4
5.54
0.76
2.89
4.21
C V (%)
12.26
7.94
7.96
8.97
9.03
6.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MCP-2/CCL8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MCP-2/CCL8 in samples. No significant cross-reactivity or interference between Mouse MCP-2/CCL8 and analogues was observed.
