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PE Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114] (AGEL0500)

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SKU AGEL0500
Product Type Antibody
Antibody Type Monoclonal Antibody
Applications FC
Reactivity Mouse
Host Species Rat
Isotype IgG2a,k
Clone M5/114
Conjugation PE
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Description

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PE Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114]

The Mouse MHC II (I-A/I-E) Monoclonal Antibody (PE-conjugated, M5/114) is a powerful tool for researchers studying immune responses and antigen presentation in mice. This monoclonal antibody, conjugated with Phycoerythrin (PE), targets the MHC class II molecules I-A and I-E in mouse samples.MHC class II molecules play a key role in the immune system by presenting antigens to T cells, initiating specific immune responses. The M5/114 antibody specifically recognizes and binds to the I-A and I-E alleles of MHC class II molecules, enabling precise detection and analysis of antigen presentation in various cell types.Ideal for immunology and vaccine development research, this PE-conjugated monoclonal antibody is validated for use in flow cytometry applications, allowing for high-resolution analysis of MHC class II expression on immune cells.

By understanding the function and expression of MHC class II molecules, researchers can gain insights into immune responses, autoimmune diseases, and vaccine efficacy in mouse models.For researchers seeking to investigate antigen presentation pathways, T cell activation, or immune responses in mice, the Mouse MHC II (I-A/I-E) Monoclonal Antibody (PE-conjugated, M5/114) from Assay Genie provides a reliable and specific tool for accurate detection and analysis.

Product Title:PE Anti-Mouse MHC II (I-A/I-E) Antibody [M5/114]
SKU:AGEL0500
Size:25µg, 100µg
Clone No:M5/114
Synonyms:H2-Ab1/Eb1;Major histocompatibility protein class II beta chain;MHC class II H2-IA-beta-psi;I-E beta MHC class II;MHC class II;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2b, κ
Isotype Control:PE Rat IgG2b, κ Isotype Control[LTF-2]
Form:Liquid
Conjugation:PE
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Swissprot:P14483 O78196
Gene ID:14961,14969
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

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