The Mouse MMP-13 (Matrix Metalloproteinase-13) CLIA Kit is a powerful tool for researchers studying matrix metalloproteinase activity in mouse samples. This kit provides high sensitivity and specificity for the accurate detection of MMP-13 levels in serum, plasma, and cell culture supernatants. Matrix metalloproteinase-13 is an important enzyme involved in tissue remodeling and extracellular matrix degradation. It plays a key role in processes such as wound healing, tissue repair, and inflammation.
Abnormal MMP-13 expression has been linked to various diseases including arthritis, cancer, and cardiovascular disorders, making it a valuable target for research and potential therapeutic interventions.With its reliable performance and versatility, the Mouse MMP-13 CLIA Kit is an essential tool for investigating the role of MMP-13 in disease pathology, biomarker discovery, and drug development. Trust AssayGenie for accurate and reproducible results in your research efforts.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-13. You can calculate the concentration of Mouse MMP-13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
101-115
101-115
Average (%)
106
108
109
1:4
Range (%)
98-115
100-112
95-110
Average (%)
105
106
101
1:8
Range (%)
87-98
97-111
99-118
Average (%)
92
105
107
1:16
Range (%)
88-102
91-104
88-101
Average (%)
93
99
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
91-104
98
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.51
98.95
398.12
42.4
98.38
397.64
Standard deviation
4.25
8.55
32.13
4.15
10.24
29.98
C V (%)
9.14
8.64
8.07
9.79
10.41
7.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-13 in samples. No significant cross-reactivity or interference between Mouse MMP-13 and analogues was observed.
