The Mouse MMP-7 (Matrix Metalloproteinase-7) ELISA Kit is designed for the quantitative measurement of MMP-7 levels in mouse samples such as serum, plasma, and tissue homogenates. This kit boasts high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications. MMP-7 is an important enzyme involved in extracellular matrix remodeling and tissue repair. It plays a key role in processes such as wound healing, tissue regeneration, and inflammation.
Dysregulation of MMP-7 has been implicated in various diseases including cancer, arthritis, and fibrosis, making it a valuable biomarker for studying these conditions and potentially developing targeted interventions. With its user-friendly protocol and robust performance, the Mouse MMP-7 ELISA Kit from AssayGenie is an essential tool for researchers looking to investigate the role of MMP-7 in health and disease.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-7. You can calculate the concentration of Mouse MMP-7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-106
97-111
93-108
Average (%)
97
104
99
1:4
Range (%)
101-116
85-99
98-112
Average (%)
107
90
103
1:8
Range (%)
102-115
86-96
92-105
Average (%)
108
91
99
1:16
Range (%)
100-114
89-101
95-108
Average (%)
107
94
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.6
461.1
1621.7
215.3
451.1
1753.6
Standard deviation
13.1
18.4
87.6
14.4
20.8
73.7
C V (%)
6.6
3.99
5.4
6.69
4.61
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MMP-7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-7 in samples. No significant cross-reactivity or interference between Mouse MMP-7 and analogues was observed.
