The Mouse Netrin-1 (NTN1) ELISA Kit is specifically designed for the precise measurement of netrin-1 levels in mouse serum, plasma, and tissue lysates. This highly sensitive and specific kit provides accurate and reproducible results, making it an indispensable tool for researchers in various fields.Netrin-1 is a key protein involved in axon guidance and cell migration, playing a critical role in neural development and regeneration. Dysregulation of netrin-1 has been implicated in various neurological disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With its advanced technology and ease of use, the Mouse Netrin-1 (NTN1) ELISA Kit is an essential tool for researchers looking to investigate the role of netrin-1 in neurological disorders and other biological processes. Order yours today from Assay Genie to advance your research efforts.
Product Name:
Mouse Netrin 1 (Ntn1) ELISA Kit
Product Code:
MODL00976
Size:
96 Assays
Target:
Ntn1
Synonyms:
NTN1L, Epididymis tissue protein Li 131P
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
91-103
97
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
