Description
Mouse Neurokinin-B PharmaGenie ELISA - Kit Information
Neurokinin B is a peptide hormone belonging to the tachykinin family, which also includes Substance P and Neurokinin A. All the peptides from tachykinin families are derived from two preprotachykinin genes - the PPT-A gene and PPT-B gene. The former encodes Substance P. Neurokinin A & Neuropeptide K, and the latter encodes Neurokinin B. Neurokinin B has shown important roles in regulating immune and neurological functions in humans. It is found in higher concentration in women suffering from pre-eclampsia during pregnancy. It is reported to stimulate the production of immunoglobulins in peripheral B lymphocytes via its NK-3 receptor. Recently Neurokinin B, along with its NK-3 receptor, has shown to play a critical role as a key neuroregulatory switcher on human puberty, governed by the brain through the release of the hormone GnRH, which starts a series of processes that ultimately leads to the production of sex hormones. Neurokinin B has shown potential clinical application as a biomarker in pre-eclampsia during pregnancy and onset of puberty.
Mouse Neurokinin-B PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS0061 |
| Size: | 96T |
| Application: | Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC. |
| Uniprot: | P55099 |
| Gene ID: | 21334 |
| Gene Names: | NKNB / TAC2 / TAC3 |
| Synonyms: | Tachykinin-3 (Neurokinin B-like protein Zneurok1) (Preprotachykinin-B) (PPT-B) [Cleaved into: Neurokinin-B (NKB) (Neuromedin-K)] |
| Target Species: | Human, Mouse, Rat |
| Compatible Sample Types: | Cell Culture Supernatants, Serum |
| Design Principle: | Competition-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.1-1,000 pg/ml |
| Sensitivity: | 3.7 pg/ml |
| Recommended Dilution: | Human: 2X, Mouse: 2X, Rat: 2X |
| Storage/Stability: | Standard, Biotinylated Neurokinin B peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. |
Kit Components:
| 1. | Pre-Coated 96-well Strip Microplate |
| 2. | Wash Buffer |
| 3. | Standard Peptide |
| 4. | Assay Diluent(s) |
| 5. | Biotinylated Peptide |
| 6. | HRP-Streptavidin |
| 7. | TMB One-Step Substrate |
| 8. | Stop Solution |
| 9. | Assay Diagram |
| 10. | Positive Control Sample |
| 11. | Capture Antibody |
| 12. | Technical Manual |
Other materials and equipment required:
The Assay Genie Mouse Neurokinin-B ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
