The Mouse NPY (Neuropeptide Y) ELISA Kit is a powerful tool for the precise measurement of neuropeptide Y levels in mouse serum, plasma, and tissue culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research endeavors.Neuropeptide Y is a key neurotransmitter that plays a critical role in regulating various physiological processes such as food intake, energy balance, and stress response.
Dysregulation of neuropeptide Y has been linked to a variety of health conditions, including obesity, anxiety disorders, and cardiovascular diseases, underscoring its significance as a biomarker for investigating these diseases and potential therapeutic interventions.Overall, the Mouse NPY ELISA Kit offers researchers a reliable and efficient means of studying neuropeptide Y dynamics in mouse models, paving the way for deeper insights into its functions and implications for health and disease.
Product Name:
Mouse NPY (Neuropeptide Y) ELISA Kit
SKU:
MOES01292
Target:
Mouse NPY (Neuropeptide Y)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NPY. During the reaction, NPY in the sample or standard competes with a fixed amount of NPY on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NPY. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NPY in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
92-108
95-108
Average (%)
106
99
101
1:4
Range (%)
90-105
93-105
95-113
Average (%)
97
100
103
1:8
Range (%)
89-103
89-102
93-110
Average (%)
95
95
101
1:16
Range (%)
85-101
94-105
93-110
Average (%)
92
100
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
97-107
102
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.5
213.0
987.5
89.8
217.2
1073.9
Standard deviation
6.3
11.3
43.5
5.0
9.1
40.8
C V (%)
6.4
5.31
4.41
5.57
4.19
3.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of NPY concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes NPY in samples. No significant cross-reactivity or interference between NPY and analogues was observed.
