The Mouse NPY (Neuropeptide Y) ELISA Kit is a powerful tool for the precise measurement of neuropeptide Y levels in mouse serum, plasma, and tissue culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research endeavors.Neuropeptide Y is a key neurotransmitter that plays a critical role in regulating various physiological processes such as food intake, energy balance, and stress response. Dysregulation of neuropeptide Y has been linked to a variety of health conditions, including obesity, anxiety disorders, and cardiovascular diseases, underscoring its significance as a biomarker for investigating these diseases and potential therapeutic interventions.Overall, the Mouse NPY ELISA Kit offers researchers a reliable and efficient means of studying neuropeptide Y dynamics in mouse models, paving the way for deeper insights into its functions and implications for health and disease.
Product Name:
Mouse NPY (Neuropeptide Y) ELISA Kit
SKU:
MOES01292
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
