The Mouse NRG-4 (Neuregulin-4) CLIA Kit is a reliable assay designed for the quantitative detection of Neuregulin-4 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Neuregulin-4 is a critical protein involved in numerous cellular processes, including cell growth, differentiation, and survival. It plays a crucial role in various physiological and pathological conditions, including cardiac function, neurodevelopmental disorders, and metabolic diseases.
The Mouse NRG-4 CLIA Kit provides researchers with a valuable tool for studying the role of Neuregulin-4 in these conditions and exploring potential therapeutic interventions.Overall, the Mouse NRG-4 CLIA Kit is a valuable resource for researchers looking to investigate the functions and implications of Neuregulin-4 in mouse models, offering precise and reliable quantitative data to support their studies.
Product Name:
Mouse NRG-4 (Neuregulin 4) CLIA Kit
SKU:
MOES00082
Target:
Mouse NRG-4 (Neuregulin 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NRG-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NRG-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NRG-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NRG-4. You can calculate the concentration of Mouse NRG-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
94-105
97-108
Average (%)
97
99
102
1:4
Range (%)
103-120
99-114
88-99
Average (%)
110
107
93
1:8
Range (%)
90-102
88-104
86-100
Average (%)
95
95
92
1:16
Range (%)
86-97
91-103
90-103
Average (%)
91
96
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
92-105
97
Cell culture media (n=5)
95-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
189.82
477.76
1746.17
172.8
442.72
1684.39
Standard deviation
17.06
53.17
114.72
21.46
50.56
117.07
C V (%)
8.99
11.13
6.57
12.42
11.42
6.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NRG-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NRG-4 in samples. No significant cross-reactivity or interference between Mouse NRG-4 and analogues was observed.
