The Mouse NSE (Neuron-Specific Enolase) CLIA Kit is specifically designed for the accurate measurement of NSE levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and precise results for a variety of research applications.NSE is an important biomarker for neuronal cells, with elevated levels indicating neuronal damage or injury. Monitoring NSE levels can provide valuable insights into neurodegenerative diseases, brain injury, and other neurological disorders.
This kit is essential for studying neuronal health and developing potential treatments for these conditions.Overall, the Mouse NSE CLIA Kit from Assay Genie is a valuable tool for researchers investigating neuronal function and pathology in mouse models, offering high-quality and reproducible results to advance scientific understanding in the field of neuroscience.
Product Name:
Mouse NSE (Neuron-Specific Enolase) CLIA Kit
SKU:
MOES00068
Target:
Mouse NSE (Neuron-Specific Enolase)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NSE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NSE. You can calculate the concentration of Mouse NSE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
101-116
96-110
Average (%)
105
108
101
1:4
Range (%)
85-99
91-103
86-99
Average (%)
91
97
92
1:8
Range (%)
88-103
100-113
93-109
Average (%)
95
107
101
1:16
Range (%)
99-113
97-111
101-117
Average (%)
105
103
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.01
148.48
334.05
55.18
149.68
337.04
Standard deviation
6.54
12.38
26.92
6.43
13.2
25.62
C V (%)
11.89
8.34
8.06
11.65
8.82
7.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NSE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NSE in samples. No significant cross-reactivity or interference between Mouse NSE and analogues was observed.
