Mouse PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) CLIA Kit
The Mouse PCSK9 (Proprotein Convertase Subtilisin/Kexin type 9) CLIA Kit is a cutting-edge assay designed for the precise measurement of PCSK9 levels in mouse serum, plasma, and tissue homogenates. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PCSK9 is a key regulator of cholesterol metabolism, playing a vital role in the modulation of LDL receptor levels and cholesterol clearance.
Dysregulation of PCSK9 has been implicated in cardiovascular diseases, making it a valuable biomarker for studying atherosclerosis and other related conditions. The Mouse PCSK9 CLIA Kit provides researchers with a powerful tool for investigating the role of PCSK9 in disease pathology and developing potential therapeutic interventions.
Product Name:
Mouse PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) CLIA Kit
SKU:
MOES00338
Target:
Mouse PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PCSK9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PCSK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PCSK9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PCSK9. You can calculate the concentration of Mouse PCSK9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-105
99-113
93-106
Average (%)
96
107
99
1:4
Range (%)
85-95
101-117
91-105
Average (%)
90
108
98
1:8
Range (%)
95-109
97-111
102-119
Average (%)
103
104
109
1:16
Range (%)
99-114
97-109
101-113
Average (%)
106
103
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
100-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.43
131.25
409.84
49.93
127.46
423.32
Standard deviation
6.13
11.79
32.91
6.23
12.84
41.15
C V (%)
12.16
8.98
8.03
12.48
10.07
9.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PCSK9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PCSK9 in samples. No significant cross-reactivity or interference between Mouse PCSK9 and analogues was observed.
