The Mouse PDGFD (Platelet-derived growth factor D) ELISA Kit is specifically designed for the accurate measurement of PDGFD levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.PDGFD is a key growth factor that plays a critical role in promoting cell proliferation and tissue regeneration, making it a valuable biomarker for studying various diseases and developing potential treatments.
By accurately measuring PDGFD levels, researchers can gain valuable insights into the mechanisms underlying conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders, paving the way for novel therapeutic approaches.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGF-D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PDGF-D. You can calculate the concentration of Mouse PDGF-D in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
87-98
92-107
Average (%)
96
93
100
1:4
Range (%)
94-107
85-98
86-96
Average (%)
99
92
91
1:8
Range (%)
87-100
81-95
89-99
Average (%)
93
87
94
1:16
Range (%)
92-104
82-91
83-94
Average (%)
98
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
83-98
90
Cell culture media (n=5)
91-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.33
105.9
455.94
47.94
109.3
454.69
Standard deviation
2.66
5.36
22.93
3.21
5.32
18.28
C V (%)
5.5
5.06
5.03
6.7
4.87
4.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PDGF-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PDGF-D in samples. No significant cross-reactivity or interference between Mouse PDGF-D and analogues was observed.
