The Mouse PK2 (Prokineticin 2) CLIA Kit is a powerful tool for the quantitative measurement of PK2 levels in mouse samples such as serum, plasma, and tissue lysates. With its high sensitivity and accuracy, this kit delivers reliable and reproducible results, making it perfect for a variety of research applications.Prokineticin 2 is a key protein involved in various biological processes, including circadian rhythms, neurogenesis, and inflammation. Its dysregulation has been linked to conditions such as sleep disorders, mood disorders, and inflammatory diseases, making it a valuable biomarker for studying these conditions and identifying potential therapeutic targets.
Overall, the Mouse PK2 CLIA Kit is an essential tool for researchers looking to explore the role of PK2 in health and disease, providing valuable insights into its effects on physiological processes and potential implications for therapeutic interventions.
Product Name:
Mouse PK2 (Prokineticin 2) CLIA Kit
SKU:
MOES00496
Target:
Mouse PK2 (Prokineticin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PK2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PK2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PK2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PK2. You can calculate the concentration of Mouse PK2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
94-108
96-110
Average (%)
93
102
104
1:4
Range (%)
102-119
90-105
90-101
Average (%)
109
96
95
1:8
Range (%)
89-100
92-107
97-112
Average (%)
95
99
105
1:16
Range (%)
91-104
97-113
93-107
Average (%)
96
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
104
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.17
316.89
991.5
106.58
314.76
1000.57
Standard deviation
12.62
24.18
82.99
10.26
32.11
66.24
C V (%)
12.86
7.63
8.37
9.63
10.2
6.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PK2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PK2 in samples. No significant cross-reactivity or interference between Mouse PK2 and analogues was observed.
