The Mouse PP (Pepsin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of pepsin levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Pepsin is an enzyme that plays a key role in the digestion of proteins in the stomach. Abnormal levels of pepsin have been associated with conditions such as gastroesophageal reflux disease (GERD) and peptic ulcers, making it a valuable biomarker for studying these conditions and developing potential therapies.
Overall, the Mouse PP (Pepsin) CLIA Kit from AssayGenie is a powerful tool for researchers looking to study the role of pepsin in mouse models and further our understanding of digestive disorders.
Product Name:
Mouse PP (Pepsin) CLIA Kit
SKU:
MOES00468
Target:
Mouse PP (Pepsin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PP. You can calculate the concentration of Mouse PP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
94-109
90-102
Average (%)
93
102
97
1:4
Range (%)
102-114
98-114
96-110
Average (%)
109
105
104
1:8
Range (%)
95-110
97-108
97-113
Average (%)
103
103
105
1:16
Range (%)
85-96
101-119
99-111
Average (%)
90
108
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
102-116
107
Cell culture media (n=5)
100-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
2.11
8.1
1.17
2.28
8.63
Standard deviation
0.1
0.16
0.6
0.14
0.21
0.85
C V (%)
9.35
7.58
7.41
11.97
9.21
9.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PP in samples. No significant cross-reactivity or interference between Mouse PP and analogues was observed.
