The Mouse PPAR Gamma (Peroxisome Proliferator-Activated Receptor Gamma) CLIA Kit is specifically designed for the quantitative determination of PPAR Gamma levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, ensuring precise and reliable results for various research applications.PPAR Gamma is a key transcription factor involved in regulating adipocyte differentiation, lipid metabolism, and insulin sensitivity. Dysregulation of PPAR Gamma is associated with metabolic disorders such as obesity, diabetes, and cardiovascular diseases.
Therefore, detecting and monitoring PPAR Gamma levels can provide insights into metabolic regulation and potential therapeutic interventions.With its user-friendly format and comprehensive instructions, the Mouse PPAR Gamma CLIA Kit is an essential tool for researchers studying metabolic diseases and exploring the role of PPAR Gamma in various physiological processes. Get accurate and reproducible data with this advanced kit from Assay Genie.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PPAR-γ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PPAR-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PPAR-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PPAR-γ. You can calculate the concentration of Mouse PPAR-γ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
86-98
94-110
Average (%)
103
93
101
1:4
Range (%)
87-98
99-115
92-106
Average (%)
92
107
99
1:8
Range (%)
100-113
90-104
84-99
Average (%)
106
97
90
1:16
Range (%)
85-100
96-114
104-119
Average (%)
91
104
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
101
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
101-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.11
235.17
700.81
100.69
236.27
681.5
Standard deviation
9.74
20.53
48.57
11.9
21.78
60.86
C V (%)
9.27
8.73
6.93
11.82
9.22
8.93
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PPAR-γ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PPAR-γ in samples. No significant cross-reactivity or interference between Mouse PPAR-γ and analogues was observed.
