The Mouse PSA (Prostate Specific Antigen) CLIA Kit is a highly sensitive and accurate assay designed for the detection of PSA levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a variety of research applications.PSA is a key protein involved in prostate function and is commonly used as a biomarker for prostate cancer.
Monitoring PSA levels can help in early detection, prognosis, and monitoring of treatment response in prostate cancer patients.This CLIA kit from Assay Genie offers a quick and convenient way to measure PSA levels in mouse samples, providing researchers with valuable insights into prostate health and disease progression. Trust Assay Genie for high-quality, reliable assays for your research needs.
Product Name:
Mouse PSA (Prostate Specific Antigen) CLIA Kit
SKU:
MOES00502
Target:
Mouse PSA (Prostate Specific Antigen)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PSA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PSA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PSA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PSA. You can calculate the concentration of Mouse PSA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
93-108
89-104
Average (%)
109
99
96
1:4
Range (%)
87-104
102-115
103-120
Average (%)
95
108
110
1:8
Range (%)
86-99
86-102
86-99
Average (%)
92
93
93
1:16
Range (%)
99-113
91-103
97-112
Average (%)
106
97
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
97
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.51
311.62
837.83
116.49
283.05
851.91
Standard deviation
11.42
35.09
79.59
13.07
27.51
63.3
C V (%)
10.43
11.26
9.5
11.22
9.72
7.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PSA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PSA in samples. No significant cross-reactivity or interference between Mouse PSA and analogues was observed.
