The Mouse PTGS2 (COX-2) Prostaglandin-Endoperoxide Synthase 2 CLIA Kit is a powerful tool for accurately measuring levels of COX-2 in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.COX-2, also known as prostaglandin-endoperoxide synthase 2, is a key enzyme involved in the inflammatory response and is implicated in various diseases such as arthritis, cancer, and cardiovascular disorders.
This kit allows researchers to study the role of COX-2 in these conditions and explore potential therapeutic interventions.Overall, the Mouse PTGS2 (COX-2) Prostaglandin-Endoperoxide Synthase 2 CLIA Kit provides researchers with a reliable and accurate tool for investigating the function and regulation of COX-2 in mouse models, ultimately advancing our understanding of inflammatory processes and disease mechanisms.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PTGS2/COX-2. You can calculate the concentration of Mouse PTGS2/COX-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
100-111
95-108
Average (%)
109
105
102
1:4
Range (%)
91-102
101-114
101-115
Average (%)
97
108
109
1:8
Range (%)
87-99
94-108
87-98
Average (%)
92
102
92
1:16
Range (%)
89-101
88-102
104-116
Average (%)
96
93
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
105
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.77
263.98
842.12
94.24
284.06
840.56
Standard deviation
11.1
30.75
66.44
10.55
19.94
64.22
C V (%)
11.97
11.65
7.89
11.19
7.02
7.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PTGS2/COX-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Mouse PTGS2/COX-2 and analogues was observed.
