Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) CLIA Kit
The Mouse RANTES (Regulated on Activation, Normal T cell Expressed and Secreted) CLIA Kit is a highly specific and sensitive assay designed for the accurate measurement of RANTES levels in mouse serum, plasma, and cell culture supernatants. This kit ensures precise and reproducible results, making it an invaluable tool for research studies in immunology, inflammation, and disease pathology.RANTES is a chemokine protein that plays a key role in recruiting immune cells to sites of inflammation and infection.
Dysregulation of RANTES has been linked to various inflammatory diseases, including autoimmune disorders, asthma, and atherosclerosis, making it a valuable biomarker for studying these conditions and developing targeted therapies.By utilizing the Mouse RANTES CLIA Kit, researchers can gain valuable insights into the role of RANTES in immune responses and inflammatory diseases, ultimately contributing to the development of new therapeutic strategies.
Product Name:
Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) CLIA Kit
SKU:
MOES00009
Target:
Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse RANTES. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse RANTES. You can calculate the concentration of Mouse RANTES in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
97-109
101-117
Average (%)
108
103
109
1:4
Range (%)
100-117
91-105
89-100
Average (%)
108
98
94
1:8
Range (%)
90-100
99-114
101-116
Average (%)
95
105
108
1:16
Range (%)
92-107
97-111
84-96
Average (%)
99
105
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
97-112
105
Cell culture media (n=5)
94-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.27
70.33
175.89
24.14
68.65
172.13
Standard deviation
2.21
5.45
18.05
2.93
7.54
10.43
C V (%)
9.11
7.75
10.26
12.14
10.98
6.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse RANTES concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse RANTES in samples. No significant cross-reactivity or interference between Mouse RANTES and analogues was observed.
