Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
The Mouse Rantes (Regulated on Activation, Normal T Cell Expressed and Secreted) ELISA Kit is specifically designed for the precise measurement of Rantes levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for a variety of research applications.Rantes is a key chemokine involved in immune responses and inflammation, playing a critical role in recruiting leukocytes to sites of injury or infection.
Dysregulation of Rantes has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and viral infections. The Mouse Rantes ELISA Kit provides researchers with a valuable tool for investigating the role of Rantes in these diseases and potential therapeutic interventions.
Product Name:
Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
SKU:
MOES00630
Target:
Mouse RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse RANTES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse RANTES. You can calculate the concentration of Mouse RANTES in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
96-111
89-104
Average (%)
93
104
95
1:4
Range (%)
99-113
85-98
95-111
Average (%)
105
91
102
1:8
Range (%)
101-114
83-94
94-106
Average (%)
107
88
100
1:16
Range (%)
98-111
84-99
98-111
Average (%)
105
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.2
288.2
866.9
94.2
312.3
800.9
Standard deviation
6.8
16.1
32.9
4.9
15.6
29.6
C V (%)
6.79
5.59
3.8
5.2
5.0
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse RANTES concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse RANTES in samples. No significant cross-reactivity or interference between Mouse RANTES and analogues was observed.
