The Mouse Renin Renin CLIA Kit is a powerful tool for researchers studying renin levels in mouse samples. Designed for accurate detection in serum, plasma, and cell culture supernatants, this kit offers high sensitivity and specificity for reliable results. Renin is a key enzyme in the renin-angiotensin system, playing a critical role in blood pressure regulation and fluid balance.
By accurately measuring renin levels, researchers can gain valuable insights into cardiovascular health and potential therapeutic targets. Ideal for a wide range of research applications, the Mouse Renin Renin CLIA Kit is a valuable asset for any laboratory studying cardiovascular diseases and hypertension.
Product Name:
Mouse REN (Renin) CLIA Kit
SKU:
MOES00053
Target:
Mouse REN (Renin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse REN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse REN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse REN. You can calculate the concentration of Mouse REN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
93-106
87-100
Average (%)
91
98
94
1:4
Range (%)
98-113
87-101
101-113
Average (%)
105
94
107
1:8
Range (%)
97-112
92-107
101-118
Average (%)
102
100
108
1:16
Range (%)
98-113
84-99
90-102
Average (%)
104
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
98
EDTA plasma (n=5)
84-97
90
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.51
189.82
721.98
100.99
185.0
671.6
Standard deviation
12.22
22.42
57.47
8.1
14.36
56.55
C V (%)
12.16
11.81
7.96
8.02
7.76
8.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse REN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse REN in samples. No significant cross-reactivity or interference between Mouse REN and analogues was observed.
