The Mouse RETN (Resistin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of resistin levels in mouse serum, plasma, and cell culture supernatants. This kit is ideal for researchers studying the role of resistin in metabolic disorders, inflammation, and insulin resistance.Resistin is a adipokine that plays a key role in regulating glucose and lipid metabolism, as well as inflammation. Elevated levels of resistin have been associated with obesity, type 2 diabetes, and cardiovascular diseases, making it an important biomarker for understanding and developing treatments for these conditions.
The Mouse RETN (Resistin) CLIA Kit offers reliable and reproducible results, allowing researchers to confidently analyze resistin levels in their samples. With its high sensitivity and specificity, this kit is a valuable tool for a wide range of research applications in the field of metabolic and inflammatory diseases.
Product Name:
Mouse RETN (Resistin) CLIA Kit
SKU:
MOES00339
Target:
Mouse RETN (Resistin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse RETN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RETN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse RETN. You can calculate the concentration of Mouse RETN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-118
90-106
92-105
Average (%)
108
97
99
1:4
Range (%)
89-101
87-102
88-100
Average (%)
95
93
94
1:8
Range (%)
90-105
100-116
99-119
Average (%)
97
108
108
1:16
Range (%)
101-114
91-103
93-110
Average (%)
108
98
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-116
106
EDTA plasma (n=5)
92-109
99
Cell culture media (n=5)
93-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.81
143.03
436.93
52.68
135.57
445.89
Standard deviation
5.25
11.94
42.69
4.75
10.19
32.64
C V (%)
10.54
8.35
9.77
9.02
7.52
7.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse RETN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse RETN in samples. No significant cross-reactivity or interference between Mouse RETN and analogues was observed.
