The Mouse SAA (Serum Amyloid A) CLIA Kit is specifically designed for the precise measurement of mouse SAA levels in serum samples. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research experiments.SAA is a key acute phase protein that is involved in the inflammatory response and is known to be elevated in conditions such as infection, inflammation, and various diseases.
Monitoring SAA levels can provide valuable insights into the progression of these conditions and aid in the development of potential treatment strategies.With its easy-to-use format and reliable performance, the Mouse SAA CLIA Kit is an indispensable tool for researchers studying immune responses, inflammatory diseases, and other related fields. Get your kit today and uncover the secrets of SAA in mouse samples.
Product Name:
Mouse SAA (Serum amyloid A) CLIA Kit
SKU:
MOES00527
Target:
Mouse SAA (Serum amyloid A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SAA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SAA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SAA. You can calculate the concentration of Mouse SAA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
92-107
92-107
Average (%)
109
99
100
1:4
Range (%)
93-105
96-113
99-117
Average (%)
99
104
107
1:8
Range (%)
97-111
98-110
98-111
Average (%)
103
104
104
1:16
Range (%)
100-114
85-97
94-111
Average (%)
108
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
101
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
2.87
7.16
0.93
3.03
7.66
Standard deviation
0.12
0.27
0.5
0.09
0.27
0.53
C V (%)
11.76
9.41
6.98
9.68
8.91
6.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SAA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SAA in samples. No significant cross-reactivity or interference between Mouse SAA and analogues was observed.
