The Mouse SCF (Stem Cell Factor) ELISA Kit is a highly sensitive and specific assay designed for the precise detection of SCF levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for various research applications.Stem Cell Factor (SCF) is a critical cytokine that plays a key role in regulating hematopoiesis, cell survival, and proliferation.
It is essential for the maintenance and expansion of hematopoietic stem cells, making it an important factor in stem cell research and regenerative medicine.With its high sensitivity and specificity, the Mouse SCF ELISA Kit is a valuable tool for studying the role of SCF in various biological processes and diseases, facilitating research in areas such as stem cell biology, cancer, and immune system regulation.
Product Name:
Mouse SCF (Stem Cell Factor) ELISA Kit
SKU:
MOES01144
Target:
Mouse SCF (Stem Cell Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SCF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SCF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SCF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SCF. You can calculate the concentration of Mouse SCF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
90-101
96-110
Average (%)
90
95
103
1:4
Range (%)
88-102
88-99
87-98
Average (%)
94
93
92
1:8
Range (%)
91-107
86-97
82-94
Average (%)
99
91
88
1:16
Range (%)
93-107
87-99
85-97
Average (%)
100
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
96-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.42
99.24
334.47
51.5
102.41
345.92
Standard deviation
2.99
4.0
17.46
3.18
4.79
15.81
C V (%)
5.49
4.03
5.22
6.17
4.68
4.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SCF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SCF in samples. No significant cross-reactivity or interference between Mouse SCF and analogues was observed.
