The Mouse SDF1 (Stromal Cell-Derived Factor-1) CLIA Kit is specifically designed for the accurate measurement of SDF1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.SDF1 is a key chemokine involved in cell migration, inflammation, and tissue regeneration. It plays a crucial role in various physiological processes, including stem cell mobilization, immune response, and wound healing.
By accurately quantifying SDF1 levels, researchers can gain valuable insights into its functions and potential implications in diseases such as cancer, cardiovascular disorders, and inflammatory conditions.Overall, the Mouse SDF1 CLIA Kit provides a reliable tool for studying the role of SDF1 in different biological processes and disease states, enabling researchers to advance their understanding and develop novel therapeutic strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SDF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SDF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SDF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SDF1. You can calculate the concentration of Mouse SDF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
84-96
101-116
Average (%)
99
91
110
1:4
Range (%)
102-113
94-111
95-110
Average (%)
108
102
101
1:8
Range (%)
88-102
94-109
94-107
Average (%)
94
100
100
1:16
Range (%)
92-106
100-113
92-108
Average (%)
98
107
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.57
320.13
939.52
113.95
308.94
855.71
Standard deviation
8.55
29.55
68.58
9.73
32.84
54.42
C V (%)
8.02
9.23
7.3
8.54
10.63
6.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SDF1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SDF1 in samples. No significant cross-reactivity or interference between Mouse SDF1 and analogues was observed.
