The Mouse SELP (P-Selectin) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of P-Selectin levels in mouse serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it a valuable tool for researchers studying inflammation, thrombosis, and vascular diseases.P-Selectin is a cell adhesion molecule involved in the recruitment of leukocytes to sites of inflammation and the formation of blood clots.
Abnormal levels of P-Selectin have been implicated in various inflammatory and thrombotic disorders, highlighting its importance as a potential biomarker for monitoring disease progression and assessing therapeutic interventions.With its ease of use and accurate results, the Mouse SELP CLIA Kit is an essential resource for researchers studying P-Selectin biology and its role in disease pathology.
Product Name:
Mouse SELP (P-Selectin) CLIA Kit
SKU:
MOES00342
Target:
Mouse SELP (P-Selectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SELP. You can calculate the concentration of Mouse SELP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
100-114
96-109
Average (%)
97
106
103
1:4
Range (%)
91-103
103-116
98-112
Average (%)
97
109
106
1:8
Range (%)
87-97
97-110
101-113
Average (%)
92
103
107
1:16
Range (%)
92-104
93-109
93-109
Average (%)
98
99
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
95-113
103
Cell culture media (n=5)
97-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.03
172.97
752.06
110.3
189.16
701.74
Standard deviation
13.24
19.27
62.65
9.14
18.75
55.72
C V (%)
12.37
11.14
8.33
8.29
9.91
7.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SELP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SELP in samples. No significant cross-reactivity or interference between Mouse SELP and analogues was observed.
