The Mouse Selenoprotein P (SEPP1) ELISA Kit is meticulously developed to facilitate the precise quantification of SEPP1 levels in a variety of biological samples. Selenoprotein P is a crucial plasma glycoprotein that plays a pivotal role in the delivery and regulation of selenium, an essential trace element vital for various physiological processes, including antioxidant defense, thyroid hormone metabolism, and immune response modulation. Perturbations in SEPP1 levels are implicated in conditions related to selenium deficiency, oxidative stress, and metabolic disorders, underscoring its significance as a biomarker in health and disease. This ELISA kit empowers researchers to elucidate the concentrations of SEPP1, offering valuable insights into its functional mechanisms and its implications in diverse health conditions.
By accurately measuring SEPP1 levels, scientists can gain a deeper understanding of selenium homeostasis and its impact on overall health, thereby paving the way for targeted interventions and therapeutic strategies to address selenium-related disorders. The SEPP1 ELISA Kit from Assay Genie is engineered with exceptional sensitivity and specificity to deliver robust and reproducible results. With a commitment to stringent quality control standards, this kit ensures reliable performance and user-friendly operation, making it the preferred choice for cutting-edge research exploring the roles of SEPP1 in biological systems and human health.
Product Name:
Mouse SEPP1 (Selenoprotein P) ELISA Kit
SKU:
AEES00237
Target:
Mouse SEPP1 (Selenoprotein P)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SEPP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SEPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SEPP1. You can calculate the concentration of Mouse SEPP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
89-103
95-109
Average (%)
93
96
103
1:4
Range (%)
96-106
100-110
83-97
Average (%)
101
105
91
1:8
Range (%)
88-94
85-96
84-97
Average (%)
91
93
90
1:16
Range (%)
83-98
86-94
96-107
Average (%)
90
90
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
95
EDTA plasma (n=5)
91-99
96
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
87.46
213.7
907.27
85.58
243.06
934.31
Standard deviation
5.18
9.68
43.1
4.37
11.01
43.17
C V (%)
5.92
4.53
4.75
5.11
4.53
4.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse SEPP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SEPP1 in samples. No significant cross-reactivity or interference between Mouse SEPP1 and analogues was observed.
